Foot and Mouth Disease Virus O Antibody (FMDV-O Ab) Test, Stable
Rapid Diagnostic Trustorthy
Application:
The Foot and Mouth Disease Virus Anti-O Antibody (FMDV-O Ab) Rapid
Test Cassette (Serum/Plasma) is a lateral flow
immunochromatographic assay for the qualitative detection of
serotype-O FMDV antibodies in swine’s or cattle’s serum, plasma.
It’s for the monitoring of FMDV-O antibody level and providing an
important reference for the vaccination plan.
| Principle: | Chromatographic Immunoassay |
| Specimen: | Serum/Plasma |
| Pack: | 10 T |
| Shelf Life: | 2 Years |
| Specificity: | 95.70% |
| Format: | Cassette |
| Reading Time: | 10 Minutes |
| Storage Temperature: | 4-30℃ |
| Sensitivity: | 97.50% |
| Accuracy: | 96.50% |
Description:
The FMDV-O Antibody Rapid Test Cassette (Serum/Plasma) is based on
sandwich lateral flow immunochromatographic assay. The test
cassette has a testing window for the observation of assay running
and result reading. The testing window has an invisible T (test)
zone and a C (control) zone before running the assay. When the
treated sample was applied into the sample well on the cassette,
the liquid will laterally flow through the surface of the test
strip and react with the pre-coated FMDV-O antigens. If there are
anti-O FMDV antibodies in the specimen, a visible T line will
appears. The C line should always appear after a sample is applied,
which indicates a valid result.
1. Collect swine’s or cattle’s fresh whole blood, and separate serum or plasma from
blood as soon as possible to avoid hemolysis. Only use clear,
non-hemolyzed specimens. Do not leave the specimens at room
temperature for prolonged periods. Serum and plasma specimens may
be stored at 2-8 °C for up to 3 days. For long term storage,
specimens should be kept below -20°C.
2. Move the test cassette from the foil pouch and use it as soon as
possible. Best results will be obtained if the assay is performed
within one hour.
3. Place the test cassette on a clean and level surface. Hold the
dropper vertically and transfer 2 drops of serum or plasma (approximately 50 μl) to the specimen well (S) of the test cassette, then start the
timer. See illustration below.
4. Read the results at 10 minutes. Do not interpret results after 15 minutes.
INTERPRETATION OF RESULTS
Positive: The presence of both C line and T line, no matter T line is clear
or vague.
Negative: Only clear C line appears.
Invalid: No colored line appears in C region, no matter whether T line
appears.
