Porcine Reproductive and Respiratory Syndrome Virus Antibody Test

Application:

​The PRRSV Antibody Rapid Test Cassette is a rapid chromatographic immunoassay for the qualitative detection of antibodies to Porcine Reproductive and Respiratory Syndrome Virus in serum or plasma.

Description:

The PRRSV Antibody Rapid Test Cassette (Serum/Plasma) is a qualitative, membrane based immunoassay with a double antigen system for the detection of antibodies to Porcine Reproductive & Respiratory Syndrome Virus in serum or plasma. The membrane is pre-coated with recombinant PRRSV antigens. During testing, antibodies to PRRSV, if present in serum or plasma specimen react with pre-coated PRRSV antigen in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with recombinant PRRSV antigens on the membrane in the test line region. If the specimen contains antibodies to Porcine Reproductive & Respiratory Virus, a colored line will appear in the test line region indicating a positive result. If the specimen does not contain Porcine Reproductive & Respiratory Virus antibodies, a colored line will not appear in the test line region indicating a negative result. To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.

How to use?

• Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
• Collect fresh whole blood, and separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, specimens should be kept below -20 °C.
• Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 75 μl) to the specimen well (S) of the test cassette, then start the timer. See illustration below.
• Read the results at 10 minutes. Do not interpret results after 20 minutes.
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