Bovine and Sheep Brucella Antibody Rapid Test Cassette (Whole
Blood/Serum/Plasma)
| Product: | Bovine And Sheep Brucella Antibody Rapid Test Cassette (Whole
Blood/Serum/Plasma) |
| Specimen: | WB/S/P |
| Shelf Life: | 2 Years |
| Reading Time: | 10 Minutes |
| Specificity: | 95.08% |
| Principle: | Chromatographic Immunoassay |
| Pack: | 10 T |
| Format: | Cassette |
| Sensitivity: | 95.70% |
| Accuracy: | 95.45% |
INTENDED USE
The Bovine and Sheep Brucella Antibody Rapid Test Cassette (Whole Blood/Serum/Plasma) is a sandwich lateral flow immunochromatographic assay for the qualitative detection of brucella antibody in bovine or sheep’s whole blood, serum or plasma.
PRINCIPLE
The Bovine and Sheep Brucella Antibody Rapid Test Cassette is based on sandwich lateral flow immunochromatographic assay. The test cassette has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample well on the cassette, the liquid will
laterally flow on the surface of the test strip. If there is enough Bovine
and Sheep brucella antibody in the sample, a visible T line will
appear.
The C line should always appear after a sample is applied, indicating a valid result. By this means, the cassette can
accurately indicate the presence of Bovine and Sheep brucella antibody in the sample.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or refrigerated (2-30 °C). The test is stable through the
expiration date printed on the sealed pouch. The test cassette must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.
DIRECTIONS FOR USE
Allow the test Cassette, specimen, buffer to equilibrate to room temperature (15-30 °C) prior to testing.
1.Collect bovine or sheep’s fresh whole blood, or separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, serum and plasma specimens should be kept below -20 °C.
2. Place the test cassette on a clean and level surface.
3.Hold the dropper vertically and transfer 1 drop of sample (approximately 10 μL) and add 2 drops of buffer (approximately 80 μL) to the specimen well (S), then start the timer. See illustration below.
4.Read the result in 10 minutes. Do not interpret results after 15 minutes.
Positive: The presence of both C line and T line, no matter T line is clear or vague.
Negative: Only clear C line appears.
Invalid: No colored line appears in C zone, no matter whether T line appears.
